Isolation and Identification of Copepods Indivuals from Kufa River Molecular Diversity Study
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Keywords:
: Jaccard's coefficient, Diaptomidae family, Diaptomus sp, NaupliusAbstract
Copepods are key components of aquatic ecosystems and can help regulate the global carbon cycle. Much attention has been paid to the species diversity of copepods worldwide, but the genetic diversity of copepods in Al kufa river is unexplored. Random amplified polymorphic DNA (RAPD) technique was used as a tool for assessing genetic diversity and species relationships among four species from Cyclopedia family Cyclops, Cyclops strenuus , Macrocyclops and Microcyclop. and Two species from the Diaptomidae family ,Diaptomus sp ,Nauplius . These samples were collected from a different region in the Kufa River in Najaf - Iraq . Three primers were used (OPC2 ,OPC8 and BH11) in Copepods species and the value of Jaccard's coefficient ranged from 0.46 to 0.06. Based on the bivariate (1-0) data and genetic similarity with the use of the UPGMA cluster method, the derogram separated the studied species. Our findings explored a high species diversity of copepods that was detected over a small geographic sampling range .Results from this study contribute to a better understanding of copepod diversity of Al Kufa river by using RAPD technique, an efficient technique for studying the molecular characterization and used for resolving relationships among copepod populations.
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