An Estimation of some DNA Repair Genes Polymorphisms APE1, XRCC1, and RAD18) in X-Ray Technicians
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https://doi.org/10.61705/g1mgfc98Keywords:
DNA repair genes, polymorphisms, APE1, XRCC1, RAD18, X-ray technicians.Abstract
The current study dealt with detection of some DNA repair genes polymorphisms in individuals worked in the X-ray department in Al-Sadr hospital city in Al-Najaf province. In this study, about 20 X-ray workers who worked in the Najaf province from 15/2/ 2023 to 15/3/ 2023, participated. Thirty people in the control group appeared to be in good condition. the present study was suggested, three DNA repair genes with common SNPs were enrolled, including X-ray repair cross-complementing group 1 (XRCC1) Arg399Gln (28152) G>A, apurinic /apyrimidinic endonuclease 1(APE1) Asp148Glu (rs3136820), and RAD18 Arg302Gln (rs373572), The data analysis showed significant association (p =0.002) of RAD18 genotyping with workers, AA was more frequented in workers than control while GG didn’t appear in cases. A significant association of XRCC1 (AG) with workers (p= 0.005), AA didn't appear in cases and GG was low frequented in cases than in control. Non-significant associations of APE1 (p= 0.2978, p =0.174) with case. Belong to work time, all genes included (RAD 18, XRCC1, and APE1) were non-significant affected by work time, at p-value of RAD 18 genotypes (p 0.581), XRCC1 (p =0.194) and APE1 (p= 0.891). The time of x-ray exposure was divided into less than 6 hours and more than 6 hours, with a Non-significant association of RAD18 (p =0.191), XRCC1(p= 0.662), and APE1 (p =0.576) with the time of exposure, the work duration also divided to two classes (more than 10 years and less than 10 years), non-significant associations were observed, RAD 18 (P= 0.888), XRCC1 (p= 0.319) and APE1 (p= 0.496). The present results concluded that there were significant associations between RAD18 Arg302Gln (rs373572),(AA)polymorphism was frequented in workers than control and XRCC1 Arg399Gln (28152) G>A with x-ray workers and non-significant association with APE1 Asp148Glu (rs3136820), on the other hand, the time, number of exposure and work period didn't associate with repair genes.
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